On foreign policies, Leader underlined that "international interaction must not make us neglect enemy's plots as some of foreign states just seek destruction of Iran", he said.
He added that Tehran "will show a very clever reaction" to the imposition of sanctions.
Ayatollah Khamenei further said Iranian officials have succeeded to cooperate with the world despite enemy attempts to isolate the nation.
Trump, who during his election campaign a year ago called 2015's nuclear agreement "the worst deal ever", signed new sanctions into law on Wednesday, along with measures against Russian Federation and North Korea.
Ray said the joint commission allowed for the Iranian view on new USA sanctions to be widely discussed.
The law also imposed new sanctions on Russian Federation and North Korea.
Based on the deal, nuclear-related sanctions on various Iranian business and financial sectors were lifted, but it put more strict limits on its nuclear program.
"And the second debate is, if these sanctions are to be implemented, how should Iran respond?"
Shutdown, restrictions hit Indian-controlled Kashmir following death of civilian
The state government has closed all the colleges and schools in wake of the students protests. A fierce gunfight followed, police sources said.
The coincidence of the endorsement ceremony with the auspicious birth anniversary of Imam Ali ibn Musa ar-Ridha brings about dignity and consolation for the loving hearts of the Iranian people and those who attended the ceremony, the Iranian Supreme Leader said.
"I recommend three major orientations: first, addressing the problems of the people..."
"Four decades of experience, in worldwide relations, reveals that the cost of surrendering to aggressive powers is far greater than the cost of standing up to them".
Rouhani will be sworn in on Saturday and then have two weeks to present his cabinet to parliament for a vote of confidence.
The diplomat also said presidents of seven countries had arrived Iran by Thursday night to take part in the ceremony.
During the event, Rouhani received his validation edict from the Leader and was officially validated into the office for the second time.
He gained 57.14% of the votes.
Rouhani, who will be formally sworn into office on Saturday in parliament, said the nuclear deal has been a sign of "good faith" by Iran and that t brought the nation respite from most hard U.N. sanctions.
His conservative rival Ebrahim Raisi came second with 38.5 per cent.