South Africa, however, bolstered by its victory in the last match, will have an opportunity to end the six-match series on an even note.
India finally won an ODI series in South Africa.
After winning the toss, South Africa put India to bat.
Shreyas Iyer has been showing the elements of an global batsman, but he has failed to strike big as he edged Ngidi to Klaasen behind the wicket just when it seemed that he was building the platform for a late innings flourish.
Kohli and Rohit ensured India had yet another fruitful second-wicket association, putting on 105 in 109 balls, though Kohli never kicked into dominant gear, as only two fours in his 54-ball 36 showed.
After that India looked well set to go way past the 300-mark with Rohit impressive after only managing 40 runs in his previous four innings in the series. David Miller (36) and Klaasen (39) were the other top contributors.
But his dismissal, for India's fourth wicket, precipitated a mini collapse as 21-year-old fast bowler Lungisani Ngidi took four wickets in quick succession to finish with figures of 4-51.
The team batting 1st has won 19 games, while the team bowling first has also won 19 times.
Incidentally, Kohli was also the leading run-scorer in the three-Test series that preceded the ODIs - the Indian captain had amassed 286 runs including a classy 153.
Oxycontin Maker Purdue Pharma Will Stop Marketing the Drug to US Doctors
Centers for Disease Control and Prevention , and the most recent figures suggest that 145 Americans now die every day from overdoses.
The Men in Blue still have a two-match cushion in the six-ODI series.
Kohli supported him, hitting his 75 off 83 balls to continue a run of red-hot form this series, which India had completely dominated until Saturday. India posted 274/7 in 50 overs, thanks to Rohit Sharma's 17th ODI hundred.
Toss: South Africa won the toss and made a decision to Field.
South Africa stayed alive in the one-day global series against India with an enthralling five-wicket win in the fourth game on Saturday.
"This obviously hurts the guys a hell of a lot so we're going to look to bounce back and at least end the one-day series on a positive note", South Africa captain Aiden Markram said.
Ngidi struck again the very next ball as Hardik Pandya suffered the same fate as Rohit and had to trudge back to the pavilion for a golden duck.
The live broadcast will begin at 4.30 pm IST.
Virat Kohli and co started the series in fine fashion, securing dominant victories in the first three contests at Durban, Centurion and Cape Town respectively to take their tally to 3-0.
It was Jasprit Bumrah who dismissed the skipper to give India the first break through.